Re: [DIYbio] Current state of smallest genome aka minimal cell?

On 04/28/2018 11:38 PM, Koeng wrote:
> Soooo right now at the BioBricks FreeGenes Project (I help lead that effort, having marketed it much yet because I'm still scaling our robots, submission->shipping, etc) we are synthesizing every single gene from Mycoplasma JCVI syn3.0, genitalium, and pneumoniae, plus all the genes from Mesoplasma florum (also the essential gene sets from Bacillus subtilis and E. coli) all codon optimized for E. coli expression (MoClo compatible). We already have the gene cloning down, so it should take us about 2 weeks to clone those 3mbps, but in the future the aim is to get protein purification automated with the opentrons so we can begin discovering all the functions and combinations of all those genes.

Please tell us more about protein purification with the opentrons.

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