Re: [DIYbio] Nanoporing a human genome

Hello,

I like your idea!

Oxford Nanopore (O.N.) long read technology is great for deciphering portions of the genome that would usually go unnoticed - such at short tandem repeats and homopolymers - and is also great for creating long contiguous sequences (or "contigs") that can then be used to align to one another more accurately.

That being said, the accuracy of base calling just isn't the same with the Nanopore read technology.

Today, most labs doing full genome sequencing are using O.N. to generate a skeleton genome map and then using Illumina sequencing to "polish" the map with more accurate sequence data.

There is a company in the United States (Texas) called "Mr. DNA". They sequence pretty much what ever you send them.

There are also numerous genomics sequencing "cores" at universities and institutes around the United States that supplement their research with funding from sequencing. UCSD and The Scripps Research Institute both have DNA sequencing cores that provide Illumina and Oxford Nanopore technologies.

-SG

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