I know NEB sells it as part of their protein expression kit... as I got one once as a part of a bunch of free products I won from them, but never used it and it was left in a university freezer that I believe experienced a power loss later. Do we need to start a collection to get you started? I have some relatively easy projects (major assumption) that I was planning to use k.lactis as the main host, so I'm interested. I couldn't figure out a better way to keep the plasmid stable in subculture aside from using the acetamide selection NEB sells in the pKlac1 plasmid.
On Sat, May 26, 2018 at 2:34 PM, Andreas "Mega" Stuermer <andreas.t.stuermer@gmail.com> wrote:
Hi, as the title says, wondering if someone had a sample to share
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-Nathan
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