Re: [DIYbio] Re: HV Power Source

On 05/01/2018 11:04 PM, James Feeney wrote:
> I think it could be used for electroporation, however the plates would have to be precise gap width, and pulse duration would not
> be very predictable at all.   (Some papers suggest that pulse duration is not critical...)

Sure, my electroporator, https://github.com/kanzure/culture_shock, uses one stage of doubling.
It's driven by a python program creating pulse trains of sequenced duty cycle into the load, so the pulse shape CAN be controlled.
It behaves like a HV DAC with a little fish-tailing overshoot if you try to make it put out
over 50 kHz. Besides being an electroporator, the circuit boards can be a HV playground for tinkerers and engineers.
I'll be announcing it here when ready to sell.

If anyone is interested in doublers, I have a bunch of commercial HV PS doubler sections I can sell. They are all nicely spaced
out and hold charge for a good while if not bled off for safety. They are quite deadly when they have enough capacity to supply
more than the milliamps needed to cause fibrillation from an arm to arm shock...and I think these do.

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