Will deionized water work, maybe? If I do 1% extraction buffer and 99% dH2O, the pH might still be stable (measure it) and there's not much NaCl to prevent binding of the partners
On Tuesday, June 19, 2018 at 1:26:52 PM UTC+2, Andreas "Mega" Stuermer wrote:
Hi guys,I want a peptide to bind to a protein.After I extracted the protein from the organism (extraction buffer 0.1M Tris-HCl, 0.1M NaCl, pH 8) I will put them into a MWCO centrifuge thing to remove small molecules with 3 kDa cut-off (everything smaller than 30 amino acids flows through the filter and is discarded).What buffer should I put the protein and the peptide in? I know they react under physiological conditions in the cytosol. Maybe I should not use extraction buffer at all and just use the cell pulp plus protease inhibitors?Looking forward to some input
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