On Thu, Jun 21, 2018 at 7:39 PM, Koeng <koeng101@gmail.com> wrote:
Yea it's been moving around a bit. As I said, under constant development still.Got a link to the reddit post? Would love to talk to them as well. The biggest issue in our lab for colony picking is the inconsistent lighting (we have windows) so making colony picking robust is more difficult than it would be to just have a simple box.
Have you thought of using NIR light? Or another method would be to pulse your light source (any color/spectrum) at a known rate (i.e. something that isn't a harmonic of 60Hz, what artificial lights might be pulsing to) and sync your camera to snap a photo on each ON & OFF and then you can subtract the OFF photo, to normalize the ON data from inconsistent ambient lighting (really you only need an OFF image every once in a while, as fast as the lighting might change... so that could be every other frame, or it could be once or twice a minute).
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