Re: [DIYbio] stable transformation of chlorella vulgaris

hello everyone 

i am currently working on a project for production of vaccine based on stable transformation of chlorella vulgaris. the plasmid size is around 6 Kb and i wanted to transformed into chlorella vulgaris using electroporation to get a stable expression of my protein of interest and also i wanna analyze the site of integration into genome.  we have bio-rad gene pulser II in our lab.

so can someone help me for the design of protocol for the transformation.

thanks in advance.

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