Re: [DIYbio] Spider Beer Overview: Making Yeast Produce Black Widow Silk


Not a fool's errand at all

On Oct 12, 2018, at 3:23 PM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:

Per the success of the "spider-goat" project, you can extrude spider silk from concentrated solution. IIRC, the quality of the resulting silk is far lower than that produced by spinnerets. Last time I looked the best and most practical route was engineered silkworms.

On 12 October 2018 22:47:33 GMT+01:00, "Daniel C." <dcrookston@gmail.com> wrote:
On Fri, Oct 12, 2018 at 12:49 PM Nathan McCorkle <nmz787@gmail.com> wrote:

Yeah, you need a spinner, and I think the supply to the spinner requires different properties than simple cell lysate/exudate (I'm guessing that has to do with purity and concentration).

So is this guy on a fool's errand?  Even if he can make yeast that makes the protein, he won't have actual strands of silk?  Or is there a way to extrude it without growing a full spider?

-Dan 


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