Re: [DIYbio] Sterilizing plant hormones


Hi Andreas!

you may ask the [PLANT-TC] group.

long time ago I made some PLANT-TC experiments in Klagenfurt
and I learned from a Netherland professional company that some
amoebe survived even hard autoclaving - they had to have it
tested by canadian specialists in order to find out.

In my naive view as a newby I think this is why many
microorganisms produce mucus - just to have some protected
encapsulation that cannot so easily be penetrated by acids and heat.

filter sterilization will not get rid of viruses but will hold back anything
larger than 15 micrometers - it will also hold back those cells
encapsulated in mucus - so my best guess is to obey the protocol.

yours, Gerald

 
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Gerald Trost
Software Developer / Programmierer
phone: +43 (0)650 2311057
skype: gerald.trost

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_________________________________________________________________________
 
 
Sent: Saturday, November 17, 2018 at 10:49 AM
From: "Andreas \"Mega\" Stuermer" <andreas.t.stuermer@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: [DIYbio] Sterilizing plant hormones
Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.

My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?

Google was remarkably unhelpful to give a yes/no answer

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