[DIYbio] in vitro CRISPR

Hi!

I remember a friend saying that the CRISPR-Cas9 protein can actually use ssDNA guides as opposed to RNA guides. Citation needed. 

Does anyone have experience with this? 

Considering this for science workshop, because if you are doing it in vitro, you don't get genetically modified organisms, and you can do it at any high school without a governmentally certified lab in Europe. 

Thanks! 

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