I'm working on the FreeGenes project (have for a while) on building another parts repository that is actually open source (iGEM simply doesn't use terms or conditions with their plasmid stocks, we're using the OpenMTA). It turns out it's actually really hard to build large quantities of parts: for about a year we messed around with in house cloning, and got pretty far, but now we're just ordering clonal DNA directly from Twist. I'm beginning to hit on toolkits that are generally useful by spreading out the number of suppliers we have rather than cleverness to clone hard things in house, so it's just taking a while. It's also just hard to scale the DNA design process.
The FreeGenes parts collections aren't out yet, but will be sooner or later. For now, if you want parts and are unaffiliated with an institution, you either know people, get them from the odin, or get affiliated.
koeng
On Wednesday, April 10, 2019 at 8:08:00 AM UTC-7, Christopher Monaco wrote:
On Wednesday, April 10, 2019 at 8:08:00 AM UTC-7, Christopher Monaco wrote:
Hi all,
I'm getting ready to gear up our synbio efforts at my DIYbio space and was looking iGEM and their biological parts registry as routes to go. It seems really difficult to have acess to the tools they offer and I'm honestly not sure if there's even a community iGEM track anymore. Based on posts on this mailing list (the most recent of which are several years old), it looks like the DIYbio community has more or less given up hope of having a working relationship with these folks. Which is a real shame because closing off access to parts from the public does not seem like any way to further the mission of innovating bioengineering.
Anyway, that long intro is to ask what are people in this community doing when wanting to perform synthetic biology? Is there another parts respository out there other than the one controlled by iGEM? Do you just have the requisite parts synthesized? I guess the big question is how can someone unaffiliated with an institution do work in this field?
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