To sum up this thread from my understanding of Matt's fears:
Start of thread:
-Recombinant DNA.
Middle of thread:
-Sequences that nature would not create hold the potential for novel functions far more detrimental than a human pathogen.
End of thread:
-Ligations you attempt that don't propagate and/or unpropogated e-coli.
This is really starting to feel like a troll. It doesn't help my trolling feelings that he implied that I should "give up this hobby" in his response to me. I find the middle conversation interesting/possible but unlikely to happen in a DIY lab. Much more likely to occur in a govt lab. This has been a journey that has lacked consistency. I also think that Matt should do more research and write up a column for a journal if he'd like to gain traction or be taken seriously.
On Mon, Jun 10, 2019 at 1:29 PM Matt Endrizzi <matt.endrizzi@gmail.com> wrote:
And the tube of ligation products that you transformed those bacteria with? And the colonies you don't pick?
And the bacteria that don't grow because the DNA you put in it was toxic to the cell in some way?
There's more than we don't know than we do know. A little humility on the part of people doing this work could go a long way to reassuring the public that not only is science a worthy pursuit, but the people pursuing it are ethical and careful.
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