Dear DIYbio,
-- I've reverse engineered a short hairpin RNA sequence that produces an interfering RNA that I want to express in human cells.
This RNAi targets myotilin, which when misfolded causes a very rare form of muscular dystrophy. Eliminating the misfolded myotilin largely reverses the disease symptoms in both mice and rhesus monkeys. No side effects from the loss of myotilin were observed.
I now have no idea how to proceed with getting AAV to express the above. The design, and manipulation of plasmids is beyond me, so I'm happy to ask a plasmid factory to do this for me, it's just that I don't know what to ask for......
Any help would be appreciated.....
Many thanks,
Simon.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2BGvnugdj7zkKHdvRyuWR9NO-%3Dgsr5PeNEJQ6ib2jQ7dyVG%3Dsw%40mail.gmail.com.
0 comments:
Post a Comment