What do you want to do? I heard of a psbA mutant that is not green (heard of it many years ago, maybe it was another gene). So it could be selection marker plus visual marker,
-- Besides that pCambia1302 is a standard go-to. They sell it online for 100 bucks I think, but someone in your country might have some as well so shipping may be cheaper
On Tuesday, August 13, 2019 at 11:49:07 PM UTC+2, Tom De Medts wrote:
On Tuesday, August 13, 2019 at 11:49:07 PM UTC+2, Tom De Medts wrote:
Hi folks!Would any of you happen to have plasmid DNA constructs that work as visible markers of plant genetic transformation, without the need for complicated equipment for screening fluorescent (GFP, DsRed, mCherry etc) protein screening OR without the need for destructive assay like the traditional GUS staining protocol ?Something likeRNAi against phytogene desaturase - bleached, white leavesand / oroverexpression of anthocyanin - purple colored plant tissueswould be idea.Please let me know. Any and all ideas welcome! Thanks, in advance.Cheers,Tom
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