[DIYbio] Re: Cleaning methods for pipette tips

Good afternoon Mr Jordan
If you have a protocol that you follow for the below, can you please share the same with me?

On Wednesday, April 13, 2011 at 4:20:57 AM UTC+5:30, Jordan Miller wrote:
sonicating in Alconox soapy water will remove almost everything biologic that might contaminate your next sample. Then rinse. This is our preferred way to reuse most of the plastics in the lab.

also, pipette tips DO survive autoclaving, so after rinsing off the alconox with distilled water you can autoclave them in their box.

Many pipette companies will send you free samples of their tips in an autoclavable box, just ask them! If you are going to reuse tips then that one sample box should last you a good long time.

jordan

On Apr 12, 2011, at 6:41 PM, Jelmer Cnossen wrote:

> Hello DIYbio-ers!
>
> I'm still a newbie at the practical lab side of biology. I'm wondering if people know [good] methods of cleaning pipette tips for re-use.
> Obviously not for the well funded labs that just buy new tips everytime, but this would reduce costs for DIYbio labs.
> I did some google searches, but so far haven't found anything.
>
> The reason I'm asking this is because I want to work towards building a cheap DIYbio lab robot,
> and having a cleaning system would lower cost and simplify the robot (No need to switch tips)
>
> Would any of these things make sense:
> - Very small scale localised autoclaving? Normal tips would melt, but maybe metal tips could work here, or something else that is temperature-resistant.
> - Chemical cleaning of tips, but I guess that if you need chemicals you might as well use
> - Remove the biological compounds using other methods, maybe sound waves (Sonification)
>
> Please contribute any crazy ideas you might have! :)
>
> kind regards,
> Jelmer Cnossen
>
>
>
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[DIYbio] Centrifuge safety PSA

Thanks for sharing! Definitely important for newbies

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[DIYbio] Re: If you were in charge of your own genome...

Super p53 mice - typo

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[DIYbio] Re: If you were in charge of your own genome...

Large animals solve cancer by having extra copies of the Central housekeeping switch off the cell - p53. That sounds like a pretty modification. Look up "suoer p53 mice"

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[DIYbio] Pulse-field electrophoresis

Hi everyone! Wondering if anyone here is working with Pulse-field electrophoresis. We want to do some research on full length p53 gene, hence we need to isolate a 50kbp fragment from a 300 kbp bacterial artificial chromosome.

Open to collaborations (or slightly paid service if necessary)

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[DIYbio] Kids doing DIY biology

Hi all 

I'm a science writer from the UK who has covered biohacking, DIY bio and biodesign for various outlets and for my next article I'm looking for examples of really young kids getting into DIY biology. I really like the idea that even schoolkids are now able to doing innovative biotech and genetic engineering projects from their homes. 

I've already spoken to a couple of really interesting young people using Amino Labs kit, one in Canada and the other in Washington DC, and I'm looking for a few more to speak to - ideally in the UK or Europe (although I know things are little more tightly regulated here making it more difficult). 

Any help would be greatly appreciated! Please message me directly if you have any info or ideas. 

All the best
Tom 

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[DIYbio] interesting Bloomberg BusinessWeek article on DNA sequencing, aging.

https://www.bloomberg.com/news/features/2019-11-13/chinese-genetics-giant-bgi-wants-to-tailor-medicine-to-your-dna

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[DIYbio] Re: If you were in charge of your own genome...

For myself, the only one I'm currently sure of is replacing my APOE4 allele with an APOE2 (other one is already APOE3), which offers relative benefits for heart disease and dementia risk, and late-age memory.

Haven't given thought to this question regarding humanity generally. The list linked above looks cool but the research seems incomplete on a per-gene basis (for genes that are mentioned, not all known effects are there), and many neural health associated genes are known to have trade-offs or conditional dependencies that make them less than straightforwardly beneficial. So y'know, don't rush into editing any of that into the global human genome unless you have an ERO stat. =P

On Tuesday, November 19, 2019 at 8:05:53 PM UTC+1, Yuriy wrote:
This is a hypothetical question.
If you were in control of the human genome, What would you add, remove, trade-in from the human genomes thus human condition?

Would the change be to improve from an ailment or to augment an already healthy body?

Would the change be on a somal or gamete forming scale?

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Re: [DIYbio] If you were in charge of your own genome...

Oh wow, Thank you Nathan.

I never realized someone has been compiling a list. 

That's a huge wishlist.

Does anyone else have anything to add?

On Wednesday, November 20, 2019 at 2:15:13 PM UTC-5, Nathan McCorkle wrote:
All of the above.

Here's a list of possible edits:
http://diyhpl.us/wiki/genetic-modifications/

On Tue, Nov 19, 2019 at 11:05 AM Yuriy <yuriy...@gmail.com> wrote:
>
> This is a hypothetical question.
> If you were in control of the human genome, What would you add, remove, trade-in from the human genomes thus human condition?
>
> Would the change be to improve from an ailment or to augment an already healthy body?
>
> Would the change be on a somal or gamete forming scale?
>
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Re: [DIYbio] If you were in charge of your own genome...

Oh wow, I never realized someone has been compiling a list. 

That's a huge wishlist.

Does anyone else have anything to add?

On Wednesday, November 20, 2019 at 2:15:13 PM UTC-5, Nathan McCorkle wrote:
All of the above.

Here's a list of possible edits:
http://diyhpl.us/wiki/genetic-modifications/

On Tue, Nov 19, 2019 at 11:05 AM Yuriy <yuriy...@gmail.com> wrote:
>
> This is a hypothetical question.
> If you were in control of the human genome, What would you add, remove, trade-in from the human genomes thus human condition?
>
> Would the change be to improve from an ailment or to augment an already healthy body?
>
> Would the change be on a somal or gamete forming scale?
>
> --
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> Learn more at www.diybio.org
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Re: [DIYbio] If you were in charge of your own genome...

All of the above.

Here's a list of possible edits:
http://diyhpl.us/wiki/genetic-modifications/

On Tue, Nov 19, 2019 at 11:05 AM Yuriy <yuriyology@gmail.com> wrote:
>
> This is a hypothetical question.
> If you were in control of the human genome, What would you add, remove, trade-in from the human genomes thus human condition?
>
> Would the change be to improve from an ailment or to augment an already healthy body?
>
> Would the change be on a somal or gamete forming scale?
>
> --
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> Learn more at www.diybio.org
> ---
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[DIYbio] If you were in charge of your own genome...

This is a hypothetical question.
If you were in control of the human genome, What would you add, remove, trade-in from the human genomes thus human condition?

Would the change be to improve from an ailment or to augment an already healthy body?

Would the change be on a somal or gamete forming scale?

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