[DIYbio] Re: DIY COVID-19 PCR test

Results and Discussion
Validation of qRT-PCR assay
The Ct value was not produced from negative control, indicating the reaction was done aseptically.

Hi Derek,

What company is that?  And how much / what's their lead time?  I'm asking as some of us from the London BioHackSpace want to be able to test ourselves and those near to us... to minimise us infecting others if / when we're infected.

Thank you,

AJP

p.s. if you're interested you can join the slack group and join the #lets-beat-corona channel , though we'll be sure to post here with any relevant info / decisions / purchases.

On Friday, February 28, 2020 at 5:19:20 PM UTC, Derek wrote:

Hey Tito,

Been doing some research on this. With the long incubation time I'd like to know when it's circulating in the community before cases are seen. Have found an rt-pcr protocol and located a company that will sell me a plasmid with part of the capsid envelope to use as a positive control. Biobasic has all the primers cheaply. Still working on a collection protocol to allow me to swab banisters, etc. A bunch of folks at the victoria makerspace are interested in helping so will eventually be documenting our steps. Happy to share any details.

Derek