Like John said, what you're calling the shock chamber is nothing more than a holder with spring loaded contacts for either side of the disposable* electroporation cuvettes. (* People have claimed getting many uses out of these by sterilizing with HCl or other obvious chemical means).
If you have a 3d printer or basic wood-shop level fabrication tools, you can easily rig something up. Just make sure it's easy/quick to remove the cuvette, since the time from shocking to adding fresh media needs to be minimal.
On Thu, Mar 5, 2020, 9:49 AM Tom De Medts <tdmedts@gmail.com> wrote:
Dear DIY Bio community,--I seek your help in identifying a used, fully functional bacterial electroporator for garage lab use.I've found a few on Ebay, but some of them are in the price range of $1000 - $3000, which is almost an order of magnitude more than my budget, which is ~ $300 - $500 including tax + shipping.Often times, I find the setup includes, if it is BioRad,the gene pulser,the capacitance extender, andthe pulse controller,but missing the shocking chamber. I wonder if the cuvette chamber is the first item in the setup to breakdown? To make things worse, the cuvette chamber aka shock chamber costs > $500 just by itself.So I wonder whether there are secret / hidden / obscure sellers, incuding but notl imited to Ebay, and LabX.com etc, from where I can buy an electroporator (BioRad preferred, but not mandatory) that is fully functional for bacterial gene transfer (not interested in eukaryotes).Thanks, and some links from my online research are listed below.Best,TomJUST CHAMBER
https://www.ebay.com/itm/Biorad-MicroPulser-Electropcrator-Shock-Chamber-Assy/143546859131?_trksid=p2485497.m4902.l9144
FULL SETUP
https://www.ebay.com/itm/BIO-RAD-Gene-Pulser-Pulse-Controller-Capacitance-Extender-plus-Chamber-shock-qz/193210236659?hash=item2cfc3a3ef3:g:c7UAAOSw7oldztU1&autorefresh=true
https://www.ebay.com/itm/BIO-RAD-Gene-Pulser-II-Pulse-Controller-Capacitance-Extender-plus-Chamber-shock/153804889929?hash=item23cf7c5f49:g:NL0AAOSwgZpduPcM
https://www.ebay.com/itm/BIO-RAD-Gene-Pulser-II-Pulse-Controller-II-Capacitance-Extender-II-Chamber-4652/263745627533?_trksid=p2485497.m4902.l9144
PARTIAL SETUPS
https://www.ebay.com/itm/Bio-Rad-Pulse-Controller-Capacitance-Extender-and-Gene-Pulser/233318965856
https://www.ebay.com/itm/Bio-Rad-Pulse-Controller-Plus-with-MicroPulser-Shock-Chamber-II/274284018442
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CANpz%3DfFOtF63H_jTv%2BanpXMh1QmzBdY6w8K_YHMU2N8Ta_rdKA%40mail.gmail.com.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9LPpeFSzkUiVzfnEvZBoZWfUyJmrBiOzzrLnj8Spm7X%2Bg%40mail.gmail.com.
0 comments:
Post a Comment