LPS is a bit too much for use as an adjuvent, I would think. And standardising the dose would be super important and super hard at DIY scale.

Generally I would say DIY is never injectable, and the skills and resources needed to do safe biomedicine are best acquired the traditional way.

On 7 March 2020 04:32:31 GMT+00:00, Dakota Hamill <dko...@gmail.com> wrote:

I'm no immunologist but I think that's how you'd give yourself toxic shock syndrome. 

On Fri, Mar 6, 2020 at 9:21 PM Michael Flynn <mfly...@gmail.com> wrote:

Often in vaccine development, in addition to target antigens that you want to raise antibodies against, an adjuvant, something that stimulates the immune system like lipopolysaccharide, must be added in order to generate an adaptive immune response against the antigen. E.coli is covered with lipopolysaccharide, which reacts strongly to complement (antibacterial factors in blood), and so would be a good candidate for an adjuvant. This line of thinking leads to an "easy vaccine" where you express the antigen in E.coli and thermally lyse the cells, dilute them to some concentration in water for injection, and there you have your vaccine. In an outbreak like coronavirus, making vaccines like this would seem to be "low risk, high reward" in that nothing bad seems likely to come of it, but you could potentially immunize against this bad outbreak.

Anyone have any actual literature on this?

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