Years back someone suggested using a restriction enzyme sample to transform bacteria and then streaking out on various antibiotics, to see if the production plasmid could be recovered as a contaminant.
Possibly it could make even more sense if you are willing to discard a miniprep-volume worth of RE mix and concentrate any contaminating plasmid first..
For best results though you'd probably want the higher end enzymes that have undergone some engineering
On 5 March 2020 17:38:01 GMT+00:00, Tom De Medts <tdmedts@gmail.com> wrote:
Hi folks,For those of us in the US, working in a DIY / garage lab setting, rather than from an incubator or MakerHacker-Space, from where can we obtain restriction enzymes?I looked at Amazon.com, and it looks like Takara Bio might have sold these at some point, but they are all unavailable at the moment.Some tye REs of interest are:
- ECoR I,
- BamH I,
- Sma I,
- Hind III,
- Sal I,
- Sac I,
- Not I etc.
Thanks , in advance.Cheers,Tom
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