Re: [DIYbio] Re: Covid vaccine.

Related to glycosylation differences in humans versus other species in which to raise antibodies I recently read an article about the Canadian Quebec company Medicago having patents for glysolyation pattern modifications in the host plants and ramping up production of massive quantities of COVID-19 antigen to be used for vaccinations with government support.
See this link: https://www.medicago.com/en/newsroom/

Ironically the host plant species is tobacco and not medicago which is the company name. medicago is a legume genus that includes the species alpha alpha.

Anyways my question was whether anyone here is familiar with what glysolylation modifications medicago has been awarded patent protection for. I am traveling with spotty internet connectivity, or I would get on a desktop and check CAMBIA's lens or WIPO's patentscope. can anyone help

This email was text to speech so please pardon complete lack of punctuation. Thank you. Stay safe.

On Tue, Apr 7, 2020 at 11:20 AM Zonie deep <deck...@gmail.com> wrote:

Never had a problem with codon usage. Bacteriophage love E. coli. Many large proteins degraded when expressed in bacteria but small pieces like this usually quite stable and highly expressed. Especially when made as fusion proteins. To express like this is routine.  Many companies have made spike protein already. 

On Tue, Apr 7, 2020, 8:58 AM Reginald Smith <rsm...@supremevinegar.com> wrote:

Ok....not sure about all this since I believe the devil is always in the details, but I have no expertise here and won't pass judgment. 

My only comment is once you have your cDNA, make sure you alter the sequence to prioritize synonymous codons favored for expression in E. Coli or whatever you are inserting this into. I don't know how far of codon usage bias or even the genetic code is between SARS-CoV-2 and E. Coli but I suspect there are important differences.

On Sunday, April 5, 2020 at 7:02:06 PM UTC-4, Zonie deep wrote:

You know if we are desperate enough it would be quite easy to make your own purified recombinant covid spike protein in bacteria. This could be used for a vaccine without too much regulatory. Protein could be made in a few weeks. Need to be sterile of course, dosage worked out. I've only immunized rabbits with these types of preps. Always get good antibodies,no harm to rabbits.

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