Hi guys!
-- Wondering the following:
If you buy liver from the store, it's been sitting on 4°C for a few days...
Wondering if you can still extract exosomes and proteins from inbetween the cells and how you would go about it.
I mean, exosomes are pretty stable for what I remember - at least they survive freezing. There's also this protein called MSA which might be the activity we want(?)
The idea here is that usually you use "pre-conditioned" medium for stem cells. -> You grow buffalo rat liver cells in tissue culture, add fresh medium and wait a few hours until the cellls released ome exosomes and proteins. Then, the hardly-used medium is removed and stored for later use, so you can grow stem cells in it
"Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells."
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