Ok, thank you!
Kind of what I assumed - maybe we'll try it out and compare within the next year or so
On Friday, May 8, 2020 at 7:46:14 PM UTC+2, Andreas "Mega" Stuermer wrote:
Hi guys!Wondering the following:If you buy liver from the store, it's been sitting on 4°C for a few days...Wondering if you can still extract exosomes and proteins from inbetween the cells and how you would go about it.I mean, exosomes are pretty stable for what I remember - at least they survive freezing. There's also this protein called MSA which might be the activity we want(?)The idea here is that usually you use "pre-conditioned" medium for stem cells. -> You grow buffalo rat liver cells in tissue culture, add fresh medium and wait a few hours until the cellls released ome exosomes and proteins. Then, the hardly-used medium is removed and stored for later use, so you can grow stem cells in it"Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells."
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