FreeGenes has been working on some gene sets, but they might not be that useful to you - (http://stanford.freegenes.org/, password: freegenes). We got a lot of MoClo compatible full genome sets, and have begun working on toolkits for individual organisms now that we've figured out processing, quality control, etc. Operations turn out to be, hard-ish? I wish we had better toolkits, and we should by now, but that's just how it is. Sorry, we're working on it.
On Monday, May 4, 2020 at 11:51:25 PM UTC-7, Dakota Hamill wrote:
-- We give away all our gene sets for free (so long as you're not shady as hell, but that's a Stanford liability thing). We also pioneered the OpenMTA, which was recently adopted by Addgene (https://help.addgene.org/hc/en-us/articles/360042153872-What-is-the-OpenMTA-), in hopes to get around that stupid "legal binder" (all our genes can be used commercially and redistributed freely). We also have a version of the OpenMTA that is unilateral to eliminate all that stupid MTA signing.
We have OpenEnzyme which might be interesting - has 44 useful off-patent enzymes, like ligases, polymerases, and restriction enzymes. All free, though they're not in expression vectors (nearly everything we make is MoClo compatible). We're also distributing a COVID-19 diagnostic toolkit, which has ready-to-use expression vectors for making the enzymes necessary to do COVID-19 tests, and we've shipped about 30 of those to 6 continents (not THAT many, but still pretty ok).
A lot of our kits I've been trying to ship to local biohacker spaces with multiple copies so they can get and share forward, eliminating the need for those centralized supply chains. We're being very comprehensive with each organism toolkit we build, so you'll be able to do virtually anything with a smallish amount of parts. Still limited by Twist's failure rate but oh well.
Hope you can find plasmids you need
On Monday, May 4, 2020 at 11:51:25 PM UTC-7, Dakota Hamill wrote:
Welcome to (barely) open science where the plasmids come with a legal binder and the "lab grade tweezers" are $79 from Fischer Sci and $1 at CVS. AddGene is like an Ivory Clubhouse outside of the Ivory Tower that you're still not invited to. Don't fret though, many of us aren't invited.Depends on the plasmid you want in regards to alternative sources.On Tue, May 5, 2020 at 1:45 AM betanic <balp...@gmail.com> wrote:I was very excited to stumble on addgene and see the plethora of plasmids they have available, then to my disspointment I find they only sell to nonprofits. I am an individual researcher, are there any similar services that I can obtain some plasmids? When I go to thermo fisher for the same plasmids, they charge about $600 for the same thing.... If anyone has suggestions or can help here that would be great. (I'm in the USA by the way)--
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