About the mineral oil that you find, I would say that could be a problema the DNase, RNase and protease present in it.
Check uct profile of the mineral oil PCR reagent: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/2/m8662dat.pdf
it says:
Product Profile
DNase, RNase and protease: None detected
Suitable for use in the Polymerase Chain Reaction (PCR).
But, maybe if you warm up the mineral oil that you found during 30 minuts at 100ºC the enzymes should denaturalize, don't they?
El sábado, 5 de septiembre de 2020 a las 5:57:03 UTC+2, Tom De Medts escribió:
Hi NicVied,Can you please share a link to this pocker PCR, thank you.Are you going to later mineral oil in lieu of a heated lid?If yes, then will you buy it from a scientific lab supplier or just from some marketplace like amazon?I found this link for the least expensive mineral oil - https://www.amazon.com/Mineral-Pure-Organic-Ingredients-Utensils/dp/B07G5KVW4G/ref=sr_1_33?dchild=1&keywords=mineral+oil+PCR&qid=1599277973&sr=8-33but I am not at all sure if this will serve the purpose...I look forward to your response.Cheers!
TdMOn Fri, Sep 4, 2020 at 3:36 AM NicVied <antoni....@gmail.com> wrote:Thanks you all, finally I bought the pocket PCR. It's really cheap but without "hot lid", old school style. I will let you know how it work!El miércoles, 19 de agosto de 2020 a las 4:36:51 UTC+2, jfo...@udel.edu escribió:It depends to some extent on how "diy" you want to be and what your skill set is... I cobbled together a serviceable thermocycler that can do a half dozen epi tubes from the cooling block from an old HP workstation I had in my attic, a cartridge heater, an arduino w/ thermocouple, and some other electronic bits and pieces I had lying around. Material cost was basically nothing, but for the time I spent tinkering to make it serviceable I could have picked up a consulting gig and made enough money to buy a nice, shiny, new one...---- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
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