Re: [DIYbio] Tecan EVO2 dispensing variation

On 10/26/20 8:53 AM, Paul Twyman wrote:
> We are running DMSO. I have replaced all syringes, 3 way valves and tips. Is there anything else that I should check or replace?

Since DMSO is a good solvent for oil based, and makes some materials swell up or dissolve, you may have deterioration of some
plastic fittings and leaking.
Is the rest of the piping path made of HDPE or Teflon or PP? If not, the parts made of anything more reactive could be
cracked/leaking.

Another way to analyze this is -- perhaps the new seals in the syringes are being affected by the DMSO while doing initial tests,
then after swelling they are stable. Try to purchase syringes with seals that are unaffected by DMSO.

--
John Griessen

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