Science

On 11/23/20 4:23 PM, 'Charles Maldonado' via DIYbio wrote:
> The moving particles are in a tube with fixed wall thickness. These particles are only moving in one direction and have a constant
> speed. So it's fine that I only measure the fluorescence directly in front of my sensor. I have to detect their presence/absence.
> I am triggering the fluorescence by illuminating the particles with light from a LED. I want to place my sensor perpendicular to
> the light source.

Since you say you don't need to know how strongly they glow, or if there are two of them clumped together, you could get away
without image processing.

" I only have to detect one fluorophore. "

Will there be a low low chance of two touching or close to each other?

If so, the sensor route is good.  You might even be able to detect when two are in the field of view by relative intensities.  Is
the light from one particle varying a lot if it is against the far wall of the tube or near wall?  Are you wanting to do any
hardware or code development, or just "full manual" proof of concept?

John

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/c79b94c9-2518-c6df-5412-4124f6bf4b03%40industromatic.com.

On 11/23/20 5:46 AM, 'Charles Maldonado' via DIYbio wrote:
> Hi all,
>
> I am developing a DIY detection sytem for fluorescence. I want to do the actual detection with a camera, but right now I am
> struggling a little bit with the selection of this camera There are so many cameras available and I don't know, which one of them
> will allow me to detect fluorescence. I guess, I need operation in low-light conditions and a high SNR. But aside from that, I
> don't know, which minimal specs I need...
> I just want to use it for detection, not for imaging.

Hi Charles,

I like Lisa's suggestion of a component very much if you are dealing with a zone of light. If you have a cell colony and want to
choose the brightest pixel out of a blob or growing cells, an image sensor (camera) would be good, otherwise if it makes a zone of
uniform light (maybe from a vial or cuvette) it can be lined up with the field of view of a sensor and get good repeatable results
when using a vial to hold "stuff" and get light out of one side of it. What kind of it are you dealing with?

Do you have a way to control where your fluorescing "thing/suspension/slide/dish" is in front of the camera/sensor? For instance
if you have a cell suspension in a particular vial with a certain wall thickness of glass you could calibrate that for a light
intensity and it wold work fine if the shape of it fits somewhere so it always aims a zone of light the same way at a sensor.

--
John Griessen
blog.kitmatic.com Albuquerque NM building lab gear for biologists

Hi all,

I am developing a DIY detection sytem for fluorescence. I want to do the actual detection with a camera, but right now I am struggling a little bit with the selection of this camera There are so many cameras available and I don't know, which one of them will allow me to detect fluorescence. I guess, I need operation in low-light conditions and a high SNR. But aside from that, I don't know, which minimal specs I need...

I just want to use it for detection, not for imaging.

Could you help me please? My max budget is around 500 USD. But the cheaper, the better :D
Thanks!

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/c5a7af8c-03b2-4bfa-af53-8a9e709c5471n%40googlegroups.com.

Hi Kristina,

I would recommend reaching out to https://www.allevi3d.com/, or https://sunpbiotech.com/ 

On Nov 19, 2020, at 10:23 AM, Kristina Girčytė <kristinagir@gmail.com> wrote:

Hi,

I am a biologist. Now I am student from European Commission media academy project based in Lithuania (Europe). I am with my team creating a science promotion episode about 3D biotechnology use in medicine. The episode might be published on TV. I am read more some articles about 3D printing projects skin and organs and I am very interested in 3D bioprinting systems how help in medicine. I am looking for an interlocutor or consultant. Could you advise us somebody who would like to give us an interview about this topic?

 Sincerely,

Kristina Girčytė 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAO7CDdv_s5aV2MvLU%2BJurHvKEGkfOssiZZL0LsqNkCRbjr9xUg%40mail.gmail.com.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/67FEA415-A366-4E54-B279-7C7265782C66%40gmail.com.

Yes, looking into it and working on sourcing ingredients for it.  It's difficult to find a reliable peptide supplier because there are many scam operations that will send you an empty tube with a nice label instead of a real product. Chitosan is ~$50 from Sigma.