" Is the light from one particle varying a lot if it is against the far wall of the tube or near wall?"
Unfortunately, I don't know, whether the light is varying or not depending on the position of the particle. I am developing the system from the scratch and there is almost no experience with fluorescence at my institute.
"Will there be a low low chance of two touching or close to each other?"
I think, the chance will be quite high...
These particles have the same emission spectrum. But my filter set is interchangeable, so separate measurements with particles with different emission spectra can be conducted.
"Are you wanting to do any hardware or code development, or just "full manual" proof of concept?"
Some code will also be part of my setup. I want to display the sensor data. My initial thought was getting the intensities of my cameras pixels, calculating the average value, normalizing it to some kind of maximum and then plotting it.
Charles
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