Re[2]: [DIYbio] Re: An update on my effort to isolate viable adipose derived stem cells for therapeutic reinjection and a call for advice

I do not suffer from atrophied adipose tissue nor anything that is
simply a matter of having insurance. I am very interested in ADSC for
multiple conditions, the most invasive being periodontal bone
regeneration to reverse gum disease gum loss and the least being
subcutaneous applications to reverse fibroproliferative conditions and
rejuvenate aged skin. None of these would be covered by any insurance
that I know of.

------ Original Message ------
From: "Dakota Hamill" <dkotes@gmail.com>
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Sent: 12/22/2020 12:12:09 PM
Subject: Re: [DIYbio] Re: An update on my effort to isolate viable
adipose derived stem cells for therapeutic reinjection and a call for
advice

>Maybe you mentioned this in your previous post but is there a specific
>reason why you want to inject yourself with your own adipose stem
>cells? Did you mention a rare disease which causes degradation of
>adipose tissue? I saw in one of the articles a rare disease which
>causes atrophy of one side of the face. I would assume all your care
>would be covered under insurance by actual doctors if this is the
>case. Wish you the best! I understand dealing with doctors can be
>frustrating sometimes.
>
>On Tue, Dec 22, 2020 at 11:48 AM Frank <fgarcia0007@gmail.com> wrote:
>>
>> Thanks for the questions - which scare me. the answer is that yes i do intend to reinject but only once I have a sound proven protocol and i have followed it successfully. I guess i have not thought of how i wold prove that I have done so.
>>
>> ------ Original Message ------
>> From: "James Clement" <clementlawyer@gmail.com>
>> To: "DIYbio" <diybio@googlegroups.com>
>> Sent: 12/22/2020 10:18:43 AM
>> Subject: [DIYbio] Re: An update on my effort to isolate viable adipose derived stem cells for therapeutic reinjection and a call for advice
>>
>> The subject line uses the term "therapeutic reinjection." Does this mean you're attempting to put the ASCs back into your body? If so, will you be testing the culture prior to injection for endotoxins? Have you planned on measuring the secretome of the cells, since ASCs have a tendency to produce proinflammatory cytokines (ILs 6, 7, 8 & 11, and TNF-alpha)? What is your intended biological endpoint for the therapy?
>>
>> On Friday, December 18, 2020 at 11:10:38 AM UTC-5 fgarc...@gmail.com wrote:
>>>
>>>
>>> So I've scoured the internet for papers and guides to nonenzymatic procedures that can be done with simple inexpensive equipment and which yields an usable quantity of fat stem cells that are alive and well.
>>>
>>> I've read a few and must say I'm not entirely sure if I understand the required steps.
>>> Each of the following describes variations of the procedure. Almost all add some fancy element in one of the steps like a commercial kit, specialized syringe with filters built in , or custom made contraptions - get a load of this "Custom-made metallic disarmable pistons, concave cell-adhesive gaskets, closed cubic unit harnessing 3 different-sized sets of blade grids on each luer-lock port, rotating canal at the center of the cube to control the flow of the lipoaspirate, http://links.lww.com/PRSGO/B308).".
>>>
>>>https://www.nature.com/articles/s41598-017-10710-6#Tab3
>>>https://journals.lww.com/prsgo/fulltext/2020/02000/a_3_step_mechanical_digestion_method_to_harvest.27.aspx
>>>https://stemcells-aesthetica.com/isolation-process-of-stem-cells-from-fatty-tissue/
>>>http://www.irvinesci.com/protocol-for-mesenchymal-stem-cell-isolation
>>>
>>> I'd like to use these to put together a protocol that uses no expensive or custom made equipment. I'd like to know if the plating and incubation is necessary and is filtering necessary? Also, is the VSF the end point or can ADSCs be isolated from VSF? any other feedback welcome.
>>
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