Maybe the easiest calculation would be like this:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3775943/
They transfect x microgrms of DNA into cells and then it gets packged into 10^8 infectious replication-deficient AAV.
So you can estimate the uptake efficiency in vitro.
On Tuesday, January 5, 2021 at 1:59:44 AM UTC+1 Andreas "Mega" Stuermer wrote:
Hi everyone!Not something for DIYbio, but as there are a lot of smart people here, I was still wondering if anyone has done this.We want to find a comparison of AAV to PEI for in vivo studies in mice.~100 Million AAV particles with GFP are equivalent to XX micrograms of DNA packaged with PEI at the optimal ratio.I guess it strongly depends on the PEI to DNA ratio; organism, injection site, ... etc. But maybe someone has seen numbers
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