Re: [DIYbio] DNA binding to column - ethanol denatured or pure?

Given the volumes needed for >98% ethanol for prep kits you'll get
hundreds to a thousand preps out of a liter. No idea what the additive
would do on a spin kit.

On Sat, Jan 9, 2021 at 1:49 PM Andreas "Mega" Stuermer
<andreas.t.stuermer@gmail.com> wrote:
>
> Hi guys!
>
> Question on purification of DNA with a DNA purification kit (or a maxiprep kit).
>
> The principle is that DNA binds to the silica columns, and then ethanol run above it so it washes away impurities.
>
> Here, pure ethanol that has not been denatured costs 30€+ per litre.. Because the government decided it's a good idea to punish also science with a heavy drinking tax on all alcohol. Infact, the chemical companies here I only see with 80€/L
>
> They add to Ethanol 1g/100L Denatonium benzoate and 1% Methylethylketon.
> Methylethylketon looks like it would evaporate easily, so that might not be an issue.
>
> Denatonium benzoate - no idea if it binds to DNA or silica under the given condition.
>
> If you want very pure DNA, what would you do? Try to get expensive ethanol?
>
>
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