Re: Re[2]: [DIYbio] How does one hire a bio lab technician for consulting?

As you get older you produce less testosterone which coincides with an increase in visceral fat , the fat stored in the greater omentum under your abdominal muscles . That's because testosterone drives calories to be stored as subcutaneous rather than visceral fat it's one factor. So when you lose testosterone fat production shifts from subcutaneous to visceral. another hormone that drops precipitously when we get older that also has a big impact on belly fat is growth hormone. 
 testosterone replacement therapy the house can improve belly fat not just by increasing physical activity and muscle mass but more directly at a lipid metabolism level.
taking testosterone later in life is a double edged sword it can be beneficial but it can also be detrimental. The key is that you do actual replacement dosing and not more. For that you really need to know what your testosterone levels are currently and if you happen to have had a testosterone test earlier in life that is good to compare because what you want to do is take a dose that elevates your testosterone to a previous level that you actually produced . if you have a choice of testosterone derivatives, , the nandrolone's have some advantages over things like cypionate and enanthate . I would take a look at the anabolic to androgenic effect ratio which is different for each derivative.


On January 13, 2021 3:24:03 PM Tom De Medts <tdmedts@gmail.com> wrote:

@ Dakota - Is your testosterone cypionate a prescription intra-muscular injection? And is it possible to procure this without a prescription?

I have stubborn abdominal visceral fat that just will not go away to the point that exercising with that objective has led to multiple ligaments tears of the knees,
at least 3 vertebral disc wear requiring future surgery, bad low-back with very limited flexibility, displacement of cervical vertebrae etc.

I've heard that testosterone injections generally help with healing better and faster, is this true?

Also, my testosterone levels were normal in my recent blood test, but I wonder whether its levels vary just as levels of most female hormones cycle up and down? Perhaps my androgen levels
seem OK in a snapshot, but not if followed as a time course?  Like some days, in the morning especially, my voice will be deeper. And other times, my  voice will sound less deep, a little more squeaky. As a non-medical biologist, I would not venture to hypothesize if these are all related or not.  So I'm just thinking aloud and throwing out these observations.

Any suggestions / advice is welcome... 
@ Frank and Dakota, this is not at all an effort to hijack your discussion. Sorry if this is going off on a slight tangent, happy to start a new thread if you wish...

Thank you, in advance.

Cheers,
TdM


On Wed, Jan 13, 2021 at 1:09 PM Dakota Hamill <dkotes@gmail.com> wrote:
Seems like you have a solid reason.  Apologies for projecting the
notion of vanity on your decisions.   Outside of anti-aging clinics or
treating rare diseases I'm not sure who offers treatments like this
with stem cells.  How you separate who is legit from a quack, no idea
either.  I'm not even sure what FDA approved stem cell treatments
there are.

I can see the allure of anabolic steroids, I'm just on testosterone
cypionate now and it's reversed all sorts of problems I've had my
entire life overnight.

Steroids wouldn't help with your bone loss and teeth problems?
They're designed to help fight osteoporosis.

On Wed, Jan 13, 2021 at 12:09 AM Frank <fgarcia0007@gmail.com> wrote:
>
> thanks for the message and for being concerned. I am familiar with body
> dysmorphia more as an observer than a suffer. waaaaay back when I was
> young I did my stint with body building and steroids. I'm lucky that it
> did not turn into an addiction for me. I have friends who did not fare
> so well. One of them , a radiologist with whom I attended medical school
> died at the age of 31 from a massive heart attack resulting from
> cardiomegaly.
> Today I am the poster child for just about every damn age-related
> degenerative condition, I had three artificial intervertebral disks in
> my neck (before they were approved) due too herniated disks. The veins
> in my legs are basically useless forcing me to wear compression socks
> always. I got Halux Valgus (bunions) in both feet almost overnight, and
> while i have no cavities,  I do have chronic aggressive periodontal
> disease  with bone loss.  There's more but i'll hold off.   two of my
> conditions have been shown to respond and even resolve with stem cell
> therapy. With periodontitis, stem cell injections right on the receding
> edge of the bone in a matrix that keeps them there for a little while
> significantly stimulates not just bone but all the lost tissues to grow.
>   That treatment is actually in practice but it costs a fortune and I'm
> about to loose 4 teeth if i don't act soon,.
> the other which i have mentioned is more of a problem with even less of
> a solution but in which stem cells have shown remarkable efficacy..
> Believe me i have no thing about wanting to inject stem cells for
> vanity. I would love to not have to go through all this trouble, So no
> danger of me doing this just for th hell of it.
>
> To your other point, I am absolutely into anti aging but in a true way,
> not just superficially. If i had the money i would have a protocol for
> myself that attacks some of the key drives and pay people to  force me
> to do things like intermittent fasting, being in bed by midnight, and
> exercising consistently. that right there is has more impact than just
> about any single drug. Then I'd buy myself a plasmapheresis machine, and
> get my hands on  4 or 5 of the molecules in this list:
> https://docs.google.com/document/d/1c79BsB8ooKkefmKLNRwTj9F1grkyrEN0_vwwuR3gFmA/edit?usp=sharing
>
> so my metric for "youthfulness" would not be things like wrinkles but
> rather LDL/HDL ratio, GFR, C-reactive protein, HBAC, blood pressure,
> physical strength, cognitive performance,  etc.
>
>
>
>
> ------ Original Message ------
> From: "Dakota Hamill" <dkotes@gmail.com>
> To: "diybio@googlegroups.com" <diybio@googlegroups.com>
> Sent: 1/12/2021 9:45:09 PM
> Subject: Re: [DIYbio] How does one hire a bio lab technician for
> consulting?
>
> >I think you have an interesting project idea from your previous posts
> >and literature research, but it seems like you'll run into a number of
> >issues.  I hesitate to ask the "why" of why you want to re-inject
> >adipocyte stem-cells back into yourself because usually people that
> >want to do something like this have reasons no one else can talk them
> >out of.
> >
> >Not judging though, because I have some serious pathological body
> >dysmorphia issues from a rare endocrine disorder, and I'm on
> >endocrinologist supervised treatment, but it never feels like enough.
> >I assume this is what it feels like to be anorexic or obsessed with
> >plastic surgery.  People see things about themselves no one else does.
> >
> >If you're dead set on it, you're probably going to have to do it
> >yourself.  That's going to make it difficult and expensive, and
> >dangerous.  You could die from an infection or immune response.  I
> >assume everyone has a "right" to self-treat themselves, but to what
> >extent another may be liable for manslaughter if it goes awry, I have
> >no idea.
> >
> >What's your end measurable of it "working"?  Never aging?  No
> >wrinkles? No cellulite? You're going to inject it all over your entire
> >body that's covered with fat tissue?
> >
> >Skip the headache/lawsuit/hospital bill of doing it yourself if you're
> >dead-set on it and talk to
> >https://www.bostonstemcell.com/stem-cell-therapy/fat-graft/  at least
> >you can sue a doctor if it goes awry.  $900-$6,000 and only one google
> >away!
> >
> >Good luck hope you find what you're looking for.
> >
> >On Tue, Jan 12, 2021 at 4:37 PM Frank <fgarcia0007@gmail.com> wrote:
> >>
> >>
> >>
> >>  As you know I am attempting to produce an sample of viable fat stem cells suitable for personal medical use, on my own, and on a budget. At this point I think I understand the salient biological and medical issues but I am lacking hands on laboratory know-how.
> >>  I looked into utilizing a community biospace but they are nonexistent close by or not interested in what I'm doing. Renting space in a biolab is too expensive.
> >>  So I was thinking that a way to accomplish this might be to hire as a consultant an experienced lab technician who has done all of the parts of what I'm attempting to do.
> >>  they would evaluate the protocol I'm gonna follow , tell me what to expect and about pitfalls, make sure i know all needed materials and equipment, and walk me through it. They could also tell me were I can use cheaper equipment alternatives .
> >>
> >>  So my question to this group: are biolab tech folks hired for this sort of thing? Does someone doing stem cell culturing at a biotech company ever moonlight to make extra money? If so is there a preferred source for such a consultant like a forum or classified?
> >>
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