Dear Frank,
In my research, we were based on cytokines mainly done by cytokine profiling array. Besides cytokines, the main SPM was Neuroprotectin D1, something that we started to deal with via detection of activity Cytochrome P450 superfamily enzymes.
I noticed in my wet work that some cytokines are known that are proinflammatory but in specific circumstances, they are strongly anti-inflammatory (pure biochemistry). Also, include ECM (Extracellular matrix) because it can provide you with false results. For example, I worked on Chondroitin sulfate-proteoglycans in neural development which makes scars in neural tissue but it doesn't stop neural stem cell development (https://www.nature.com/articles/s41598-018-32203-w).
Sincerely,
Marko Komlosh
Marko Komlosh
On Sun, Feb 21, 2021 at 4:13 PM Frank <fgarcia0007@gmail.com> wrote:
--Thank you for responding to my question. When you worked with SPMs, do you remember which one's you used? How were they obtained? Did you purchase them from a research supply company or make them yourself?Frank------ Original Message ------From: "Marko Komlosh" <markokomlosh@gmail.com>Sent: 2/20/2021 7:03:40 PMSubject: Re: [DIYbio] Resolvins, lipoxins, ..Dear Frank,I didn't use SPMs but investigate their activity on neural stem cells in chick embryos, during my Ph.D. studies before dropping out from the Hebrew University of Jerusalem.My method was based on a cytokine profiling array. Link: https://www.fullmoonbio.com/product/cytokine-antibody-array/My conclusion is that SPMs are great for recovery but you need to investigate how the Extracellular matrix affects the delivery of SPMs.Also, Duckweed is the best source of Omega-3. The collection of duckweed can be found here: http://www.duckweed.ch/contakt/?lang=enSincerely,
Marko Komlosh--On Sun, Feb 21, 2021 at 12:11 AM Frank <fgarcia0007@gmail.com> wrote:Anyone here have any experience using, compounding, or synthesizing SPMs( specialized pro resolving mediators) such as resolvin E1?
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