Re: [DIYbio] Re: Start up

I will be using blood mainly to extract plasma and isolate DNA and specific proteins.

On Wednesday, March 24, 2021 at 5:11:41 PM UTC+3 jlund256 wrote:

S. Almo,

You received some good replies, but I think the first question has to be, what techniques are you performing with the tips and tubes?  If your experiments are microbiological, the labware would need washing or rinsing, and then sterilization is key.  If you are doing DNA extraction, and then PCR or enzymatic techniques with DNA, then washing and rinsing will likely be fine--things don't really *need* to be sterile, more important is removing bulk contaminants and DNA, and a bleach treatment will destroy DNA.  If you are working with RNA, then more strenuous steps are needed, etc.  So think about what you are doing, and what type(s) of dirt or contaminant you need to remove.  In some cases, dishwasher or equivalant washing is enough. 

It was common to use glass pipets before plastic became cheap, and they would be cleaned with a bleach treatment followed by several rinses in water / deionized water.  You may find instructions in general lab practice books or try googling around.  Plastic will not hold up for as many cycles as glass.

Cheers,

Jim Lund

On Tuesday, March 23, 2021 at 12:22:28 AM UTC-5 S. Almo wrote:

Hello

I want to be able to reuse my tips, tubes and needles. as i am mainly using those to train for biological techniques at home using my bento lab. i do not have the money to buy more tips and tubes. is there a way i can autoclave at home or just deep wash them.

Any suggestions?

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