Hi Almo,
PART 1. LOGIC OF RE-USE
Your cleaning protocol depends essentially on 2 things:
1. what did you already use the tips for?
2. what do you intend to use it for, next time?
Because you mentioned Bento Lab (link), I am assuming you were referring to the integrated PCR machine, centrifuge, gel electrophoresis apparatus.
So I am assuming your pipet tips were used for nucleic acid application.
So if you used it for PCR setup first, and want to use it for protein work next, and your protein samples will be OK despite some residual nucleic acid contamination,
then your whole time, money and other resources spent in decontamination might be worth it...
But if you used the tips already for PCR and wish to use it again for a different PCR reaction, then you should be ready for unexpected results,
even if you think your decontamination was performed perfectly. If you are willing to risk such an outcome, or if it does not bother you, then no worries...
PART 2. AUTOCLAVING
Autoclaving at 121C for 20mins is the best approach to get rid of microbial contamination,but not at all the preferred method for getting rid of certain chemicals / biopolymers, e.g. DNA.
Yes, autoclaving is independent of external altitude, pressure, temp etc. since the pressure chamber is self-contained, and yes, the weight on the pressure cooker lid determines when pressure is released.
Depending on the wattage of the stove, you will need to play around with the setting so that the duration of your 121C exposure is neither too short, nor too long of a duration, but as close to 20 mins as possible.
One way to know if your autoclaving was too weak is to use relatively inexpensive autoclave tape ~ $10 for a roll at amazon.com
It will however not tell you if your autoclaving is for too long a duration. For that use your cheap but trusted timer...
I do not autoclave extremely temperature sensitive reagents or chemicals, but when my media contains sugar(s), I try to make sure I do not start to smell caramelization.
PART 3. SOME USEFUL INFO ONLINE
For some idea of what is and what is not possible, you may refer to the tables 1 and 2 at this ThermoFisher link:
PART 4. ADDITIONAL NOTES - WATER & DETERGENT
Water - You will need distilled water for any rinses - I do not buy this, but purchased a water distiller from eBay (Made in China) and a pH meter cum conductivity meter to make sure it is close to pH 7.0 and as close to 0 µs/cm.
Detergent - And generally, for most mol. biol. applications, you will also need some special lab-application detergent, usually phosphate free ones, like the ones sold by Alconox (powder or liquid work well). I would not use general dish or dish-washer or laundry detergent or bath soap etc.
PART 5 - RANDOM IDEA
Have you considered contacting a larger DIYlab in your area that may be ordering a larger bulk, and who may be willing to re-sell smaller aliquots to members / customers such as yourself?
Good luck!
Tom
On Tue, Mar 23, 2021 at 1:22 AM S. Almo <suha.almohammadi@gmail.com> wrote:
HelloI want to be able to reuse my tips, tubes and needles. as i am mainly using those to train for biological techniques at home using my bento lab. i do not have the money to buy more tips and tubes. is there a way i can autoclave at home or just deep wash them.Any suggestions?--
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