Re[2]: [DIYbio] Substitute for calf serum?

9:33 PM |

Thanks James or responding to my question. Your answer made me curious as I find it hard to believe that there could only be one product on the market for anything . I found what appears to be a bunch of products marketed as substitutes for calf serum with some interesting advantages. Here are a couple. What do you think of these? Are they calf serum alternatives

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. C Rauch, E Feifel, EM Amann, HP Spötl… - ALTEX-Alternatives to …, 2011 - altex.org

Human autologous serum obtained using a completely closed bag system as a substitute for foetal calf serum in human mesenchymal stem cell cultures. N Mizuno, H Shiba, Y Ozeki, Y Mouri… - Cell biology …, 2006 - Wiley Online Library

… AB serum and thrombin‐activated platelet‐rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. A Kocaoemer, S Kern, H Klüter, K Bieback - Stem cells, 2007 - Wiley Online Library

------ Original Message ------

From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>

To: diybio@googlegroups.com

Sent: 4/11/2021 3:07:16 PM

Subject: Re: [DIYbio] Substitute for calf serum?

That is the holy grail of tissue culturing. If you find a magic elixir with growth hormones, salts, proteins, enzymes, and a desired pH you would be a wealthy person!

On Apr 11, 2021, at 2:52 PM, Frank <fgarcia0007@gmail.com> wrote:

Can anyone please tell me if I can use a substitute when a cell culturing protocol calls for calf serum? specifically, a substitute that does not involve cruelty to animals. is it possible to use my own blood serum?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/qVcVf5ZnPp4/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/732C4289-C0F5-47FF-B3B5-B6FE01B23FBA%40gmail.com.