Re: [DIYbio] Purchasing DNA synthesizer

On 8/9/22 13:13, Koeng wrote:
> @Abizar Yep
>
> @Dan awesome! I'll contact you about that.
>
> @Bryan let's definitely talk!
>
> > making gene-length fragments is pretty standard and inexpensive these days and is offered by a spectrum of commercial groups
>
> Over 200x price difference between genes and oligos. Oligos are about 100-1000x more expensive than they could be with current
> technology. I am aware of current commercial groups - I used to run the FreeGenes Project and oversaw a few million base pairs of
> synthesis from Twist.
>
> > The idea of a chip-based oligo assembler has been around for a while but to my knowledge no one has been able to get it to work
> well
>
> Genscript uses it in production. Already have reached out to Drew Hall + some of the Avery folks, since they're jumping on since
> the patent is expiring from custom array (just like me)

I'm finally getting moved into Albuquerque and would like help with this project since the culture shock electroporator failed to
launch. Culture shock needs plastic housings to be a complete usable system with safety testing, and I've gotten some parts to
begin developing a low pressure slow molding method that will help launch low volume electronics enclosures inexpensively and
certain kinds of low tolerance starting moldings for mechanical systems. Reducing the costs of prototype plastic molded things is
a long, big project though, and won't make money to pay the shop rent for a while... I've been building a side-hustle of trading
junk from national labs to pay that rent, which is working well. Got junk?

The culture shock control circuitry is a compact 7x6cm of pcb area that runs micropython with about 20 I/O lines, 2 USB ports,
A2D, flat flex cables to a 2nd pcb that could be anything. That could be a nice start for an oligo assembler control and it's
freepublished on github. Plus I have silicon chip layout experience that could help plan out how to attack doing a microfluidic
chip, or design parts of it to fab and test. The culture shock push-pull HV power supply might even be useful for generating
volts for parts of an electrowetting charge coupled droplet mover "lab on a" microfluidic chip -- it just wouldn't need to be so
high voltage.

Meanwhile, I'm in Stockholm this week if there's anything I can do to help while there...

--
John Griessen
industromatic.com Albuquerque NM building lab gear for biologists

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