Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)

Hi Kit,

Please any recipe with the amount? How many grams of each component?

I don't have an autoclave so I would use a pressure cooker.

Could I use whey protein instead of tryptone?

Thanks,

Markos

you could also use kitchen agar, nutritional yeast, salt, and tryptone (if you can source it) to make some LB plates - just mix everything together and autoclave it, pour some plates and store them in the fridge until you're ready to use them. Give the kids each three plates. Have the kids breathe on or touch two of them, and then add a teaspoon of bleach to one of the two. in a day you'll be able to see colonies on the plate with no bleach, and no colonies (hopefully) on the plate with bleach. It's a little more involved than the yeast version Simon suggested, but it might be a bit more engaging - it lets them go through an experiment and record results - plus get some exposure to agar plates - which are just a lot of fun and the colonies from just air and hands often end up being pretty and colourful.

On Sunday, August 13, 2023 at 5:40:01 PM UTC-7 Simon Quellen Field wrote:

It is fairly easy to show the activity of yeast in a sugar water solution.

Add bleach, and the bubbles stop.

I suspect bubbling chlorine gas through it would have the same effect, but that is not something I would try with twelve-year-olds.

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On Sun, Aug 13, 2023 at 5:14 PM Markos <mar...@c2o.pro.br> wrote:

Hello,

I'm organizing a citizen science course with diy activities about water
for young people aged between 12 and 16 years old.

I want to include an activity to demonstrate the disinfectant action of
chlorine, and I need to find an activity that uses affordable materials
and culture media and a protocol that is easy for participants to
replicate with kitchen utensils.

But as a Chemist I ask a help to suggest examples on web so I can save
time searching on Google.

Thanks,

Markos

www.c2o.pro.br

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