Re: [DIYbio] $50 camera good for microscopy with machine vision code included

The software might be nice, but the Canon has higher resolution, lower noise, higher video speeds, and a larger sensor.

That won't matter if all you need is 50 micron (or even 1 micron) resolution. But I've been getting close to 0.2 micron resolution using a Canon T3i (a fairly old camera) and a homemade microscope (I also use an excellent phase-contrast research microscope and get similar resolution but with much less aberration).

When controlled by my computer, I find the Canon to be quite suited to microphotography. You look at the computer monitor, and click on the shutter icon to take the picture. It's pretty friendly.

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On Sat, Jan 31, 2015 at 5:42 AM, John Griessen <john@industromatic.com> wrote:
I was telling Nathan how flex circuits like are used in LCD screens for desktops of a few years ago
are a commodity process of making layers with 50 micron width and space between and might be
repurposed to fabbing microfluidics inexpensively.  I took a flatbed scanner photo of the flex circuit
that could not get down to its resolution, and emailed that, and
offered to send him a piece to look at under a microscope,
since I did not have any good digital camera for microscope photos.

I was saying how I'd take the lens off of a Canon powershot for that, but it still has a crummy user interface
for microphotography, which triggered him telling me about this:


https://www.kickstarter.com/projects/botthoughts/openmv-cam-embedded-machine-vision

It's got a perfect user interface for microphotography -- python programmed machine vision
programs that can recognize features in a scene.  Just add a little problem-specific
development and you have a cell counter program.  Well, add that not-yet-existing, accurate and inexpensive
plastic molded microgrid to drop on your samples then top with a cover slip, *then*
hit the go button of the counting program...

John

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Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

there is nothing to get alarmed about mammalian cell culture until it's being used up on human beings ...


No, that TOTALLY doesn't sound like the beginnings of a Bio-weapon lab. 

On Sat, Jan 31, 2015 at 7:08 AM, Sunil Phani <consultsunilphani@gmail.com> wrote:
hello friends, 
 i respect your gathered concerns and there is nothing to get alarmed about mammalian cell culture until it's being used up on human beings ...
    and i thoroughly understand the intention behind cautioning me.. i am actually feeling very encouraged and  will be continue to pursue my passion ....
    now i am actually looking into adaptable  approaches  to produce Recombinant Factor VIII using pichia pastoris is a species of methylotrophic yeast. 
    Pichia is widely used for protein expression using recombinant DNA techniques.
    i sincerely believe that you ppl and Diybio will continuously advise me  in this regard and guide me to accomplish my goal rather than plotting the scopes of Diybio 

    "If your actions inspire others to dream more, learn more, do more and become more, you are a leader...."
  
   and in my view Diybio is a leader and savant ppl like you made this group a leader in all aspects ...

   friends pls through some light on pichia pastoris and its pros and cons 

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