Hi everyone!
I was wondering the following:
During a miniprep, you first lyse the cells with sdoium hydroxide and then neutralize everything with aetic acid.
Now a question came up that I never thought about: Will this not denature the DNA? During the high pH of NaOH there will be a lot of OH- groups floating around interacting with hydrogen bonds?
Will the DNA not be partly or fully single-stranded then?
Will it fully reaneal very fast, or not? will there be non-covalently joined and partly overlapping plasmid strands?
Not that it really matters for routine applications but I find it very interesting and would be interested if anyone has thought about this, knows literature about it or knows the right keywords.
Thanks!
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