Re: Get a plasmide - buy? share?

100uL to 1mL is great, but you do *also* need lower than this; I'd
recommend 2-20uL, because you can always pipette a few times to reach
60uL or 80uL. Being careful not to touch the destination fluid before
returning to the source fluid, of course; that would be
back-contamination. Bad news for things like PCR.

On 22/12/11 10:20, Mega wrote:
> I just ordered an adjustable pipette in the range of 100-1000 ul.
> (Because the plasmids are 200ul). Is that size correct, or shall I get
> another one of the sizes below?
> (I hope to be able to use it for all kind of experiments with
> bacteria. Would be 10-100ul better?
>
> 0.1-2.5μl
> 0.5-10μl
> 2-20μl
> 5-50μl
> 10-100μl
> 20-200μl
> 50-200μl
> 100-1000μl
> 200-1000μl
> 1000-5000μl
>
>
>
>
>
>
>
> On 22 Dez., 10:19, Cathal Garvey <cathalgar...@gmail.com> wrote:
>> As with what Averie said, freezing is easy with DNA. Just put it in the
>> freezer, surround with thermal mass, and leave it alone.
>>
>> However, if you'll be using the DNA within the month, it's probably best
>> *not* to freeze it..*if* it comes in "Tris-EDTA" buffer. This is
>> commonly shortened to TE. TE buffer protects DNA from nucleases and
>> maintains a pH that enhances stability for extended periods at 4C
>> instead; no freezing required.
>>
>> The reason to opt for no-freeze for short periods is that DNA is harmed
>> by freeze and thaw, so you don't want to be freezing it today only to be
>> thawing it for use tomorrow.
>>
>> On 21/12/11 22:39, Mega wrote:
>>
>>> When I get the plasmids, how long can I store them in the freezer
>>> (-20 C)?
>>> Or do I have to store them at -80 C?
>>
>>> Do I have to add something to the plasmids so they don't get damaged
>>> by freezing??
>>
>> --www.indiebiotech.com
>> twitter.com/onetruecathal
>> joindiaspora.com/u/cathalgarvey
>> PGP Public Key:http://bit.ly/CathalGKey
>

--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey

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