Re: Get a plasmide - buy? share?

I just ordered an adjustable pipette in the range of 100-1000 ul.
(Because the plasmids are 200ul). Is that size correct, or shall I get
another one of the sizes below?
(I hope to be able to use it for all kind of experiments with
bacteria. Would be 10-100ul better?

0.1-2.5μl
0.5-10μl
2-20μl
5-50μl
10-100μl
20-200μl
50-200μl
100-1000μl
200-1000μl
1000-5000μl

On 22 Dez., 10:19, Cathal Garvey <cathalgar...@gmail.com> wrote:
> As with what Averie said, freezing is easy with DNA. Just put it in the
> freezer, surround with thermal mass, and leave it alone.
>
> However, if you'll be using the DNA within the month, it's probably best
> *not* to freeze it..*if* it comes in "Tris-EDTA" buffer. This is
> commonly shortened to TE. TE buffer protects DNA from nucleases and
> maintains a pH that enhances stability for extended periods at 4C
> instead; no freezing required.
>
> The reason to opt for no-freeze for short periods is that DNA is harmed
> by freeze and thaw, so you don't want to be freezing it today only to be
> thawing it for use tomorrow.
>
> On 21/12/11 22:39, Mega wrote:
>
> > When I get the plasmids, how long can I store them in the freezer
> > (-20 C)?
> > Or do I have to store them at -80 C?
>
> > Do I have to add something to the plasmids so they don't get damaged
> > by freezing??
>
> --www.indiebiotech.com
> twitter.com/onetruecathal
> joindiaspora.com/u/cathalgarvey
> PGP Public Key:http://bit.ly/CathalGKey

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