I would use the microscope for bacteriology. The standard dishes are about 10cm (100mm) across. However, it also depends on what you are using your microscope for. Unless you can get a good image at 400x, you aren't going to see individual bacteria, and even at that level they are about the size of a grain of salt. The SOP in my lab is to just take a sample from the dish and prepare a slide, not to look at the plate itself because of contamination and practicality issues. If you ware making something more like a dissecting scope, for looking at colony morphology, I would say that you would want to have something that can access any part of the round plate--because you never know where the colony will be. Otherwise, just make it slide-sized.
What kind of magnification are you getting?
I've recently been thinking about microscopes. My thought has been to
make a kit for a webcam style microscope, inverted of course, because
we're concerned with observing actual working cells not dead and
mounted ones. The only thing that is really an issue is creating a
stage for such a system (optics having already been solved for the
most part by the guys working on stuff like the ps3 eye microscope,
etc.) I think I've finally cracked the issue of an inverted stage that
would be build-able with simple parts, and I'm looking for some input.
Assuming we're only talking about working surface area, how much space
would you need on a microscope stage? What sizes of culture flasks/
petri dishes does everyone use? If you could, post two numbers, an
idealized size that would fulfill your wildest dreams (if you're the
type who dreams about this stuff, lol, I know I am), and the smallest
space you could make due with realistically. Thanks for your
participation, I'll try to put up some pictures/scans of design
sketches some time soon, I've just started learning google sketch, so
no 3d models as of yet, just pen and grid paper drawings.
Jonathan Nesser
jonathan.nesser@gmail.com
diybioandneurosci.blogspot.com
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