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I have access to a 5 axis cnc machine at uni so if I can source some
Ti it mayyyy be possible

On Feb 29, 6:24 pm, John Griessen <j...@industromatic.com> wrote:
> On 02/29/2012 09:49 AM, Richard Proctor wrote:
>
> > whether the material in 3D printing can really deal
> > with those kind of forces.
>
> It's a quick calculation -- 200,000 * weight of half the part that
> extends to the full radius is the force.  Say the weight is .1 gram.
>
> Force is 20kg
>
> For extruded plastic with some voids or sintered plastic with some voids
> it could only hang together if in a sphere solid shape.  The cross section
> with 20kg force pulling it apart would be maybe 25 mm squared area if solid.
>
> Since it is a hollow thin shape to hold a removeable tube,
> answer is no.  Area might be as low as 5mm square area.
>
> Does sound like an app for Ti metal.

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could you build a chamber yourself using thick scrap metal?

DNA is the molecule of life, so its darn stable as long as no nucleases attack it.

Those speeds/forces are needed for ultracentrifuge.

Titanium is what the ultracentrifuge Tories here use, but it holds ~50ml tubes, not eppendorfs (which under those forces could crush themselves)

Actually I do mean single nucleotide differences in 0-1000 total nucleotides

Depends on what you mean by electrical method (label-free?), but yes.
The problem isn't sensing single nucleotide length differences, the
problem is sensing relevant single nucleotide length differences in
hundreds if not thousands of bases.

On Feb 29, 3:02 am, Nathan McCorkle <nmz...@gmail.com> wrote:
> Do you think some electrical method could sense single nucleotide DNA
> length differences in a femtoliter scale channel? (1micron * 1micron *
> 2microns = 2 femtoliters)... 1 micron electrodes are easily made with
> standard microfab today.
>
> I know we've talked about a few methods on here before, capacitance
> comes to mind... or maybe impedance
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics

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On Wed, Feb 29, 2012 at 10:49 AM, Richard Proctor
<richardmproctor@gmail.com> wrote:
> im going to be working on cathals dremelfuge. ive found a US company
> called Portescap who manufacture very high RPM brushless motors that
> can hit in excess of 70,000 RPM or 200,000 G .

Are those speeds/forces really necessary?

-Dan

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On 02/29/2012 09:49 AM, Richard Proctor wrote:
> whether the material in 3D printing can really deal
> with those kind of forces.

It's a quick calculation -- 200,000 * weight of half the part that
extends to the full radius is the force. Say the weight is .1 gram.

Force is 20kg

For extruded plastic with some voids or sintered plastic with some voids
it could only hang together if in a sphere solid shape. The cross section
with 20kg force pulling it apart would be maybe 25 mm squared area if solid.

Since it is a hollow thin shape to hold a removeable tube,
answer is no. Area might be as low as 5mm square area.

Does sound like an app for Ti metal.

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Hmm,

200,000g would probably mean Ti rotors and an armour plate centrifuge  chamber. If you really want that scale of speed and can manage with small volumes ie ~100uL consider using compressed air instead (a la Beckman airfuge). Only one moving part in the fuge, and you can hit 500,000g. Still going to need a Ti rotor and armour plate though...

Are there any provisions for keeping heat to a minimum, i.e. cooling chamber, vacuum? Ed K

" http://www.sciencedaily.com/releases/2012/02/120228190922.htm "

@codonAUG Dr. Crislip, the author of the article, seems to be in the
business of skeptical criticisms of science and probably has something
to say about everything in medicine. as with any therapeutic
approaches, he makes some valid point regarding the safety and
potential risks associated with probiotics and the lack of scientific
evidence supporting some of the health claims made by those who are in
the business of manufacturing and selling probiotics, which are hardly
regualted if marketed as dietary supplements.

but what i think he fails to admit, unsurprisingly, is the potential
benefits of probiotics if studied and engineered adequately to deliver
health benefits with reduced short- and long-term side effects. this
is why we do science to enable ultimately such capacities. the gut is
a complex system that is largely responsible for regulating what goes
into our bloodstream/body. the ability to engineer this system to
afford health benefits has a broad application in health and medicine
and can potentially lead to management of a host of health problems.

rahim

On Feb 29, 9:46 am, CodonAUG <elsbe...@gmail.com> wrote:
> I am a bit wary of probiotic hype.  But science based medicine does a
> better job than I do in explaining.http://www.sciencebasedmedicine.org/index.php/probiotics/

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http://www.ichineseflashcards.com will help you learn Chinese
(Mandarin) faster by using flashcards with pictures, thanks

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@romie, awesome. i am too more generally interested in the overall
health. probiotic applications in obesity is just my current project
of interest. lets talk off-line. i'll email you.

On Feb 29, 2:16 am, romie <ryrol...@gmail.com> wrote:
> I'm really interested in the idea of modified got bacteria. I don't
> really care about the obesity issue compared to overall health but I
> think the problems of integrating something new into such a complex
> environment is a shared problem. I'm not in your area but can pursue
> this idea at LA biohackers.
>
> Romie
>
> On Feb 28, 9:11 am, naiverahim <rahim.ba...@gmail.com> wrote:
>
>
>
>
>
>
>
> > Recently I've been really interested in the idea of designing 'good
> > bacteria' to do even more good for the gut. I've conceived of a way to
> > design Bifidobacteria (with 2 genes --> 2 enzymes) to destroy fat
> > before it's absorbed into the body or excreted in feces. This would be
> > a really cool designer probiotics that would benefit so many people,
> > particularly those on high fat diets (obesity). Taking Bifidobacteria
> > longum as a supplement has been shown to resist diet-induced obesity
> > and mitigate metabolic syndrome in rodents but of course the effects
> > are marginal. My goal is to shift metabolism towards fat degradation
> > in these designer probiotics to deliver appreciable benefits. It'd be
> > nice to have someone to bounce ideas with. Let me know if your
> > interested so I can send you some good literature on this.
>
> > I'll be doing much of the work between City College and GenSpace in
> > Brooklyn. Anyone in the area?
>
> > Bam!,
> > Rahim

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@ Nathan, here i am talking about destroying fatty acids (chemical).
Here is a decent paper studying the effect of reduced fat diet on body
weight and glucose tolerance; its a long-term study.

http://care.diabetesjournals.org/content/24/4/619.long

I understand that carbs are a better target but I have not yet
conceived of a way to dealing with carbs (although i will look into
the suggestions made in this thread). Feel free to join the effort.

On Feb 28, 4:03 pm, Nathan McCorkle <nmz...@gmail.com> wrote:
> On Tue, Feb 28, 2012 at 3:27 PM, naiverahim <rahim.ba...@gmail.com> wrote:
> > @Nathan needless to say, fat -> obesity -> metabolism syndrom/diabetes
> > (problem).
> > you mentioned studying obesity to fight hunger? that's an interesting
> > and probably more noble approach worth exploring but the aim here is
> > to 'destroy' fat.
>
> I'm sorry if I misunderstand, but are you talking about destroying the
> condition of 'being fat' or the chemicals known as 'fat'?
>
> As far as I know, eating fat produces ketone bodies, whereas eating
> sugar and carbs spike insulin which triggers fat synthesis. Destroying
> 'fat' chemicals in the gut doesn't sound as effective as destroying
> sugars and carbs.
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics

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im going to be working on cathals dremelfuge. ive found a US company
called Portescap who manufacture very high RPM brushless motors that
can hit in excess of 70,000 RPM or 200,000 G .

My main concern is whether the material in 3D printing can really deal
with those kind of forces.

The balance must be that the thing is light enough to not cause the
motor to lower its RPM but be stable enough to not cause eppendorf
bullets :-s

thoughts anyone....i'll be running FEA analysis on selection of
polymers in the next week.

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Thanks!!

On 29 Feb., 11:27, Cathal Garvey <cathalgar...@gmail.com> wrote:
> In T.E. buffer, DNA is pretty stable for months at 4c. Indeed, some schools of thought suggest that freezing is more harmful, if using buffers containing salt (like TE).
>
> In water, far less stability is expected, but you'll probably still get transformants.
>
>
>
>
>
>
>
>
>
> Mega <masterstorm...@gmail.com> wrote:
> >Hello,
>
> >I was wondering how long I could still use the plasmids I have.
>
> >They were stored around 2 month in the freezer (-20°C) and now some 6
> >weeks in the fridge (~7°C).
>
> >Will they degrade? And, if they degrade, when only half of them will
> >not be degraded (you can work with the half available, although you'll
> >get smaller yields).
>
> >Best
>
> >--
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I am a bit wary of probiotic hype. But science based medicine does a
better job than I do in explaining.
http://www.sciencebasedmedicine.org/index.php/probiotics/

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