Re: [DIYbio] Positive control for GMO foods?

Hi Bugs

Biorad has a GMO explorer kit with a refill that contains positive
controls. Not sure how hard it is to order.
Here is the manual for the kit www3.bio-rad.com%2Fpdf%2FBE_AN_RTGMO_v4.pdf
and the order number for the refill is as follows
166-2501EDUGMO Investigator Kit Reagent Refill Pack, includes PCR
master mix, GMO primers, plant PSII primers, GMO positive control DNA,
PCR molecular weight ruler, Orange G loading dye

Cheers
Brian Degger
On Wed, Feb 1, 2012 at 5:17 PM, Bugs <bugs@binny.eu> wrote:
>
> Hi folks,
>
> I'm about to order some PCR primers that should let me detect the CaMV
> 35s promoter, a viral promoter that's very commonly used in making GM
> crops for food use. The idea, obviously, is a PCR-based test for foods
> containing GMOs. Er, or crops that just happen to have been infected
> by that virus, but I think it's limited to the cabbage family.
>
> I'm struggling to think of a positive control that I can use in my
> assay. I found a list of approved GM crops for the EU [1], but it's a
> bit opaque. Does anyone have any pointers toward GM crops or GMO-
> derived foods that I can buy in the UK?
>
> Many thanks,
> Bugs
>
> [1] http://ec.europa.eu/food/dyna/gm_register/index_en.cfm
>
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transitlab.org
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