Probably one of the last question before monday transformation:
In a protocol I read that after you have heat shocked the eppendorf-
tube, and after cooling it and adding lb medium, and after waiting for
some 20-30 mins:
You have to take 100uL of this tube and plate it on agar. -> Now: You
wait some minutes and then get rid of the liquid (the bacteria are
said to be soaked up by the medium.) Yet it sounds strange to me
because in that liquid there should still be many bacteria thus
getting lost!!
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