Re: Back to Transformation! (Was Re: [DIYbio] Re: Which microscope?)

Are you doing calcium chloride or peg/mg? You only need heat shock for calcium chloride.

I performed another peg/mg transformation yesterday and I appeared to get transformants again. It's a great method!

Mega <masterstorm123@gmail.com> wrote:

>Probably one of the last question before monday transformation:
>
>
>In a protocol I read that after you have heat shocked the eppendorf-
>tube, and after cooling it and adding lb medium, and after waiting for
>some 20-30 mins:
>
>You have to take 100uL of this tube and plate it on agar. -> Now: You
>wait some minutes and then get rid of the liquid (the bacteria are
>said to be soaked up by the medium.) Yet it sounds strange to me
>because in that liquid there should still be many bacteria thus
>getting lost!!
>
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