Re: [DIYbio] Miniprep without EDTA

Answer: probably, but leave to lose for longer, and expect smaller yields.

Also, technical note: Apoptosis is a deliberate form of tidy cell suicide found in multicellular or at least eukaryotic organisms. Bacteria and archaea sometimes show evidence of orderly suicide, but it's not apoptosis. When they die by other causes and burst open messily, it's called 'lysing'.

Mega <masterstorm123@gmail.com> wrote:

>Was at a swimming pool store. In former times it was used, but now they
>sell nano powder.
>
>
>I put the glowing bacteria into a marmelade jar containing some 200-300
>mL of LB-Medium. It was glowing for some days but now it's not glowing
>anymore... I assume most of the bacteria are dead. Can I still use them
>for miniprep, or are the plasmids destroyed when doing apoptosis???
>
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