What do you mean by 'leave to loose'? not shaking it to keep the bacteria out of solution?
would it be much better to do a new liquid culture, 3mL or so, in a 50 or 100 mL tube? How can I make sure that they get enough oxygen without contamination? alu foil?
Good News: I asked @university, if I could get some KCl and d.earth. Now I have several gramms of both.
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Re: [DIYbio] Miniprep without EDTA
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