Re: [DIYbio] Favorite Tools for Aligning Multiple Sequences

When I was doing this last year, I downloaded the list of REs from
promega and NEB, then did a set-intersection with those lists and the
vector MCS+RE sites NOT PRESENT. That gave me a list of enzymes that I
could use for engineering, and I then just sorted by price to pick the
cheapest ones.

On Mon, Jun 25, 2012 at 8:27 PM, L <lakopa.palumbo@gmail.com> wrote:
> Hey there everyone,
> I'm looking for a way to quickly create protocols for transformation of
> artificial yeast chromosomes. Got lots of ideas, the means to try them all,
> and now I'm just looking into what restriction enzymes I'll need. Confused
> by BLAST, any help using that would be awesome.
>
> Thank you!
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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