Re: [DIYbio] Fwd: [atxhs-discuss] Resistor/arduino-based thermocycler

with 3 minutes per 12-40 cycles, thats 15-4.5 seconds per cycle, which
is unrealistic, since you generally need at least 5-15 seconds just to
melt the DNA. If this is a capillary setup then maybe I could be lead
to believe this works, but you'd also need a really high temp ramp
rate, which all of the biotech industry is shooting for.

I'm not saying this is a bad project, not at all, but your claims must
be realistic.

On Tue, Jun 26, 2012 at 12:28 PM, Bryan Bishop <kanzure@gmail.com> wrote:
>
> From: Matt C.M. <mcmancuso@gmail.com>
> Date: Tue, Jun 26, 2012 at 11:09 AM
> Subject: Re: [atxhs-discuss] OpenPCR, we should get one maybe?
> To: atxhs-discuss@googlegroups.com
> Cc: Bryan Bishop <kanzure@gmail.com>
>
>
> A member of another hackerspace and I have been diligently working on a
> resistor based thermocycler that's arduino controlled.  Its in the final
> stages of testing now, total cost is ~$50-75 depending on the arduino and
> how fancy you want to get with the details.  It has a wood laser cut box,
> and the block is milled out of a 1X3X3" block of aluminum, cooling is via a
> simple PC fan, heating is by resistors powered through an SSR with 120V AC
> so there's no bulky/expensive power supply needed.  It runs 3 minutes per
> complete PCR cycle(you usually run 12-40 cycles in a full reaction).  If
> there's interest I can work on getting another demo model built to show off
> at our hackerspace.
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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